StaffScientist-iPSCgeneticmanipulation

Do you want to help us improve human health and understand life on Earth? Make your mark by shaping the future to enable or deliver life-changing science to solve some of humanity’s greatest challenges. **The Opportunity:** The [Cellular Genomics Programme](https://www.sanger.ac.uk/programme/cellular-genomics/) is seeking a highly motivated staff scientist to lead wet lab work in the [Lee-Six group](https://www.sanger.ac.uk/group/lee-six-group/). The group is focused on studying the earliest stages of childhood cancer by investigating the conditions under which cells with particular cancer-driving mutations gain a selective advantage over their healthy neighbours. The applicant will have the opportunity to be embedded in a large community of experimental and computational scientists with access to world-leading computational and experimental facilities at the institute. **About the Team:** Henry Lee-Six is a paediatric pathology registrar. His previous work has been focused on somatic mutations in normal tissues. He is starting a new group in the Cellular Genomics Programme with the aim of understanding the interplay of somatic mutations and development in childhood cancer. A major goal of the group is to investigate the relative contribution of mutation identity, cell state, and microenvironmental context in determining whether a mutant cell progresses to form a tumour. The group pursues this line of research by studying samples of children’s tumours and normal tissues with cutting-edge spatial transcriptomic platforms coupled to different mutation-calling technologies such as laser capture microdissection and duplex sequencing. These data are used to build models of what determines a cell’s selective advantage, which are in turn queried to suggest perturbations to a cell’s state or microenvironment that might change the relative fitness of a mutant clone. These _in silico_ predictions are then tested _in vitro_ using iPSC and cell-line models. The group is a mixtu