University of Washington
Metabolism, Endocrinology and Nutrition
ResearchScientist/EngineerAssistant
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“Research Scientist/Engineer Assistant at University of Washington. Skills: Experiment and Assay Development, Animal work, Tissue Processing and Cell culture. Assist in the preparation and processing of genomic DNA, RNA, and cDNA, including support for real-time PCR workflows. Apply molecular biology techniques (e. g. , PCR, qPCR) to analyze gene expression and identify signatures in experimental and control samples”
Industry & Context.
willing to work with mice
What They're Looking For.
Must Have
Bachelor's degree in Biology, Biochemistry or related Scientific field, less than one year of relevant Pancreatic islet regeneration and diabetes research experience, willing to work with mice
Nice to Have
Prior hands-on experience with animal work including handling of large animal colonies, breeding and genetic screening of diverse mouse strains, handling and treatment of diabetic mice, At least 1-year hands-on experience in cell biology, molecular biology, and/or biochemistry, applied to Endocrinology and Diabetes Mellitus research, Experience with Metabolic Assays and protein analysis methods (e. g. , Western blot, ELISA), Experience with data analysis tools (e. g. , Excel, GraphPad Prism, or similar), Ability to quickly learn new laboratory techniques and adapt to evolving research needs
What You'll Do.
Assist in the preparation and processing of genomic DNA
including support for real-time PCR workflows
Apply molecular biology techniques (e. g.
qPCR) to analyze gene expression and identify signatures in experimental and control samples
Support protein-based assays
including protein extraction
and gel electrophoresis
Perform and help optimize ELISA and metabolic assays
Contribute to the development
and troubleshooting of experimental protocols
Perform routine mouse handling and husbandry
and monitoring animal health
Assist with breeding colony management
and coordination of experimental cohorts
Ensure compliance with institutional animal care and use protocols and maintain accurate documentation
Perform immunohistochemistry and immunofluorescence staining
including tissue preparation
and imaging preparation
Maintain and perform sterile mammalian cell culture techniques
including cell plating
and basic assay support
Assist with tissue collection
and sample preparation for downstream analyses
Maintain accurate and detailed laboratory records
including experimental data and protocols
Support lab operations
Assist with routine maintenance of laboratory equipment and ensure adequate stocking of consumables
and collaborative laboratory environment
How You'll Work.
Team & Collaboration
collaborative laboratory environment
Communication Scope
communication skills
Full Job Description
**Job Description** **The Division of Metabolism, Endocrinology and Nutrition (MET) has an outstanding opportunity for a Research Scientist/Engineer Assistant position to join the Crisa Laboratory.** **About this Opportunity** Reporting to Dr. Laura Crisa, the Research Scientist/Engineer Assistant will support studies performed and conducted in the Crisa laboratory at the University of Washington South Lake Union campus. The field of research this position is engaged in is Pancreatic islet regeneration and diabetes. **Key Responsibilities** _**Experiment and Assay Development (40%)**_ * Assist in the preparation and processing of genomic DNA, RNA, and cDNA, including support for real-time PCR workflows. * Apply molecular biology techniques (e.g., PCR, qPCR) to analyze gene expression and identify signatures in experimental and control samples. * Support protein-based assays, including protein extraction, pull-down assays, and gel electrophoresis. * Perform and help optimize ELISA and metabolic assays, ensuring accuracy, reproducibility, and proper controls. * Contribute to the development, optimization, and troubleshooting of experimental protocols. _**Animal work (30%)**_ * Perform routine mouse handling and husbandry, including tagging, tailing, genotyping, and monitoring animal health. * Assist with breeding colony management, including tracking, recordkeeping, and coordination of experimental cohorts. * Ensure compliance with institutional animal care and use protocols and maintain accurate documentation. _**Tissue Processing and Cell culture (20%)**_ * Perform immunohistochemistry and immunofluorescence staining, including tissue preparation, sectioning support, and imaging preparation. * Maintain and perform sterile mammalian cell culture techniques, including cell plating, media changes, and basic assay support. * Assist with tissue collection, processing, and sample preparation for downstream analyses. _**Other Duties (10%)**_ * Maintain accurate and detaile
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